Z01 Central imaging facility for advanced light and correlated light and electron microscopy

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27

  • B01
    • Walz
    • Kammerer
  • B02
    • Köttgen
  • B04
    • Bergmann
  • B06
    • Lausch
    • Schmidts
  • B07
    • Lienkamp
    • Arnold

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29

  • Z01
    • Nitschke
    • Eimer
  • Z02
    • Brox
    • Ronneberger
Roland Nitschke

Roland Nitschke

Principal investigator of

Stefan Eimer

Principal investigator of

The central facility Life Imaging Center (LIC) (head: R. Nitschke) and Electron Microscopy (EM) platform (head: S. Eimer) are both located in the Center of Systems Biology Analysis (ZBSA), and will as one platform provide state-of-the-art microscopy resources, and develop cutting edge methods (super resolution, advanced 2-Photon microscopy, automated intelligent time-lapse recording, correlated light and electron microscopy and 3D EM reconstructions) for the CRC projects. Z01, while maintaining the existing equipment, will provide the necessary add-ons and develop customized experimental solutions, techniques and adaptable algorithms for object recognition, tracking and signal quantification as required by most of the CRC projects. Z01 will provide these services in tight collaboration with Z02 (Ronneberger). Standard operation procedures will be developed to improve overall data quality and reproducibility. For all participating scientists and students of the CRC, courses will be offered on a regular basis for basic and advanced microscopy as well as special courses on microscope techniques (FRET, FRAP, spectral un-mixing, ratio-imaging, photo-manipulation, super-resolution stimulated emission depletion (STED)-microscopy), data analysis, visualization of large 2D - 5D data sets, deconvolution, and the methods for particle, cell and object tracking and tracing. By combining super resolution light microscopy and EM via high pressure freeze (HPF) sample preparation, we will establish correlative microscopy (CLEM). LIC and EM together will support sample preparation for CLEM. The EM part of the platform will develop new high pressure freeze (HPF) sample preparation methods of kidney tissue for EM as well as for super-resolution microscopy. This improved sample preparation will also allow large-scale 3D EM reconstructions by serial sectioning and EM tomography of kidney tissue. Precise knowledge of the global ultrastructure and morphology will facilitate the quantitative profiling of normal and mutant/disease tissues. This provides a seamless and robust workflow from the micrometer to the nanometer scale to analyze the function of genes mutated in hereditary kidney disease.